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KMID : 0545120100200030609
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Journal of Microbiology and Biotechnology
2010 Volume.20 No. 3 p.609 ~ p.614
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Cultivation of Spirulina platensis using pig wastewater in a semi-continuous process
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Chaiklahan Ratana
Chirasuwan Nattayaporn
Siangdung Wipawan
Paithoonrangsarid Kalyanee
Bunnag Boosya
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Abstract
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The effluent from anaerobic digestion contain organic nitrogen and phosphorus which are both required for growth of Spirulina platensis. Effluent (20%) from the Up-flow Anaerobic Sludge Blanket (UASB) from a pig farm supplemented with 4.5 g/L sodium bicarbonate (NaHCO3) and 0.2 g/L urea fertilizer (46:0:0, N:P:K) was found to be not only as a suitable medium for the growth of Spirulina platensis but also as a low cost alternative. Cost calculation showed that this medium is 4.4 times cheaper than modifized Zarrouk’medium. The average productivity of a semi-continuous culture grown under outdoor conditions in a six liter scale and a 100 L pilot scale were 19.9 g/m2/d and 12 g/m2/d, respectively. In addition, the biomass of organisms grown in UASB effluent contained approximately 57.9% protein, 1.12% gramma-linolenic acid and 19.5% phycocyanin. The average rates of bicarbonate, total nitrogen and phosphorus removal were 380 mg/L/d, 34 mg/L/d and 4 mg/L/d, respectively.
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KEYWORD
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Spirulina platensis, Pig waste, Nitrogen, Phosphorus
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